Mapping the human genome would require localizing each of its genes and sequencing it would require determining the exact order of the nucleotides making up each gene. Even the intermediate goal of mapping the genome presents significant computational challenges. The workshop will be concerned with algorithmic problems arising from mapping and sequencing, for example problems involving fragment assembly, particularly in the presence of noisy data, and approximate string matching. We expect to have sessions on the double digest and partial digest problems, large-scale DNA sequencing by hybridization and the shortest superstring problem, new approaches to DNA mapping and sequences, computational support of large sequencing projects (databases, sequencing accuracy, automation), and open problems in DNA mapping and sequencing. The topics of the workshop were chosen to be closely tied to the issues being considered in our Algorithm Implementation Challenge. The workshop will be used to kick off the Challenge and we will devote considerable time in the workshop to implementation issues.